The structure of volcanic cristobalite in relation to its toxicity; relevance for the variable crystalline silica hazard
© Horwell et al.; licensee BioMed Central Ltd. 2012
Received: 11 July 2012
Accepted: 31 October 2012
Published: 19 November 2012
Respirable crystalline silica (RCS) continues to pose a risk to human health worldwide. Its variable toxicity depends on inherent characteristics and external factors which influence surface chemistry. Significant population exposure to RCS occurs during volcanic eruptions, where ashfall may cover hundreds of square km and exposure may last years. Occupational exposure also occurs through mining of volcanic deposits. The primary source of RCS from volcanoes is through collapse and fragmentation of lava domes within which cristobalite is mass produced. After 30 years of research, it is still not clear if volcanic ash is a chronic respiratory health hazard. Toxicological assays have shown that cristobalite-rich ash is less toxic than expected. We investigate the reasons for this by determining the physicochemical/structural characteristics which may modify the pathogenicity of volcanic RCS. Four theories are considered: 1) the reactivity of particle surfaces is reduced due to co-substitutions of Al and Na for Si in the cristobalite structure; 2) particles consist of aggregates of cristobalite and other phases, restricting the surface area of cristobalite available for reactions in the lung; 3) the cristobalite surface is occluded by an annealed rim; 4) dissolution of other volcanic particles affects the surfaces of RCS in the lung.
The composition of volcanic cristobalite crystals was quantified by electron microprobe and differences in composition assessed by Welch’s two sample t-test. Sections of dome-rock and ash particles were imaged by scanning and transmission electron microscopy, and elemental compositions of rims determined by energy dispersive X-ray spectroscopy.
Volcanic cristobalite contains up to 4 wt. % combined Al2O3 and Na2O. Most cristobalite-bearing ash particles contain adhered materials such as feldspar and glass. No annealed rims were observed.
The composition of volcanic cristobalite particles gives insight into previously-unconsidered inherent characteristics of silica mineralogy which may affect toxicity. The structural features identified may also influence the hazard of other environmentally and occupationally produced silica dusts. Current exposure regulations do not take into account the characteristics that might render the silica surface less harmful. Further research would facilitate refinement of the existing simple, mass-based silica standard by taking into account composition, allowing higher standards to be set in industries where the silica surface is modified.
KeywordsCristobalite Volcano Respiratory health Crystalline silica Volcanic ash Soufrière Hills Quartz Variable hazards Regulations
Exposure to respirable crystalline silica (RCS) has been known for centuries to be detrimental to health, and is linked to respiratory diseases such as silicosis, lung cancer and tuberculosis. However, when classifying crystalline silica as a Group 1 carcinogen, the IARC Working Group recognised that silica dusts are variably hazardous and that their carcinogenicity may depend on inherent characteristics of the silica, and/or external factors which affect the particle surface and biological activity . The UK Health & Safety Executive assessed the potential of RCS to cause silicosis and concluded that all forms of RCS dusts of occupational relevance have this potential but that, again, the fibrogenicity will be influenced by the physicochemical characteristics of the dust .
Crystalline silica occurs as five main polymorphs with quartz, cristobalite and tridymite being more reactive and cytotoxic than coesite and stishovite . Quartz is the most common mineral in the continental crust and is the most commonly-encountered polymorph. Occupational exposure to the other polymorphs is more unusual but cristobalite exposure occurs in the ceramics industry through conversion of quartz in industrial furnaces , and through crystallization from amorphous diatomaceous earth during calcination . In vitro experiments have suggested that cristobalite and quartz are comparably cytotoxic, inflammogenic and fibrogenic [5–8].
In this paper, we investigate the structural side of the ‘structure-toxicity relationship’ for RCS generated during volcanic eruptions. Volcanoes are a major source of natural silica and 9% of the world’s population lives within 100 km of a historically active volcano . The discovery of substantial quantities of RCS in volcanic ash from both the 1980 eruption of Mt St Helens, USA and the eruption of Soufrière Hills volcano (SHV), Montserrat, West Indies (1995 onwards), resulted in widespread concern regarding exposure to volcanic ash over prolonged durations [10, 11]. As volcanoes can mass-produce RCS, in close association with other minerals during eruptions, this presents a unique opportunity to characterise mineralogical factors which could influence respiratory toxicity.
Formation of cristobalite in volcanic environments
Fragmentation of the lava produces freshly-fractured mineral surfaces, a factor which is known to increase the surface reactivity and pathogenicity of quartz [16–20] but the effects of which are less well characterised for cristobalite. Horwell et al.  showed that the surface reactivity of SHV volcanic ash is substantially reduced in aged, weathered samples, in comparison to fresh ash, and is enhanced in ground samples.
Dome-forming eruptions are often long-lived (e.g. SHV has been erupting since 1995) so it is imperative that the respiratory hazard of volcanic cristobalite is characterised. Although cristobalite has comparable toxicity to quartz, far fewer epidemiological and clinical studies have been carried out on industrial cristobalite exposure to support the laboratory conclusions, mainly because of the limited workplace exposures specific to cristobalite (e.g., pottery and refractory brick workers are usually co-exposed to quartz and cristobalite ). The diatomaceous earth industry, however, produces cristobalite from amorphous silica during calcination of diatomite, rather than a conversion from quartz to cristobalite as is the case with ceramics. We have found 24 epidemiological and clinical studies on diatomaceous earth workers in the literature; most have found a dose-response association between cristobalite exposure and lung cancer mortality, non-malignant respiratory diseases (NMRD) mortality and silicosis e.g. [22–25].
At both SHV and Mt St Helens, in vivo (inhalation and instillation) and in vitro toxicological studies were carried out to determine the likely outcomes of inhaling cristobalite-laden ash reviewed by [26, 27]. Differing study designs made comparisons challenging; however, in general, the ash was not found to cause the rapid in vivo or in vitro response expected of a crystalline silica-rich dust e.g. [28, 29]. Of the 19 studies on Mt St Helens ash (9 in vitro; 6 instillation and 4 inhalation studies), the exceptions were inhalation studies where very high doses of ash (50-100 mg m-3) were administered over long durations (up to 24 months), causing fibrosis and lesions [30, 31]. For SHV, it was concluded that the ash may pose a similar health risk to mixed coal mine dust . This conclusion was based on in vivo studies carried out over relatively short time periods< 49 weeks e.g. , and one must be cautious in interpretation of these data as there is a mean latency period for chronic silicosis of around three decades, depending on exposure . A risk assessment of the long-term, cumulative exposure to cristobalite at SHV concluded that, assuming continuing volcanic activity, the risk of silicosis for gardeners (highest occupational exposure) after 20 years of continuous exposure is 2 – 4% , based partly on the toxicology data. Overall, evidence suggests that the ash represents a relatively low hazard. Given the quantities of RCS in the ash, we sought to determine the structural factors that could be masking the toxicity of volcanic cristobalite.
The variability of the RCS hazard in workplaces is still a key issue, especially as regulations take a ‘one size fits all’ approach to crystalline silica . Studies on the RCS hazard, such as the current structural work on volcanic ash, will add to the growing weight of evidence that inherent characteristics and external factors can be measured, which may provide an opportunity to refine RCS exposure limits to take such factors into account.
Potential factors affecting toxicity
Crystal composition. The reactivity of particle surfaces could be reduced due to co-substitutions of Al and Na for Si in the cristobalite structure as the crystals form. Cristobalite has an open lattice structure which readily permits substitution of Si4+ by Al3+ with charge balancing by other cations such as Na+ or K+ .
Heterogeneous surface. During dome collapse, fragmentation of dome lava is unlikely to result in the production of fresh cristobalite crystal surfaces. Cristobalite ash particles may have fragments of other volcanic phases (plagioclase, glass, salts and other vapour-phase precipitates) inter-grown or adhered to their surfaces which will decrease the surface area of cristobalite available for reactions in the lung. Both feldspar and glass are Al-rich. The grain boundary between large (> 10 μm) ‘fish-scale’ cristobalite patches and vitreous groundmass is often diffuse, with cristobalite appearing to penetrate and merge with the groundmass . In addition, sub-micron-scale ‘feathery’ cristobalite crystallites, along with plagioclase feldspar, form as devitrification patches within groundmass glass .
Annealed surface. The surface of cristobalite particles could be occluded by an annealed glass layer (known as a Beilby layer ). Alternatively (or, in addition), during formation, if crystallization begins above the solidus when pores are still plastic (e.g. ) or during fragmentation, cristobalite particles may become coated in a nano-scale layer of amorphous volcanic glass.
Silicate dissolution. The mixture of cristobalite and other silicate and glass particles, in a heterogeneous dust, may affect the hazard. Proximity of cristobalite to soluble phases such as glass, following lung deposition and phagocytosis, could affect the RCS surface.
Sample summary with information on crystalline silica features observed by Horwell et al. 
Date of collapse/eruption
Date of collection
Crystalline silica type
174 k.a. BP
Dome lava; very dense, grey/brown
Ancient sample from old complex
✓ but rare
400 a BP
Dome lava; Dense, red/brown
Ancient simple from Castle Peak
Juvenile block; vesicular; light grey
From dome collapse deposit
Juvenile block; frothy, light grey
From dome collapse deposit
✓ but rare
Dome lava; Dense; Green/dark grey
From dome collapse deposit
Dome lava; Vesicular; pale grey
From 'Boxing Day' collapse deposit
1/8/03 – 15/11/03?
Dome lava; Dense; pale grey
From dome collapse deposit (full collapse)
Dome lava; dense; pale grey
From dome collapse deposit
Dome lava, dense, altered, red
From dome collapse deposit
Respirable (< 4 μm) fraction
Bulk ash from which cristobalite separated
The extensive collection of well characterised dome rock and ash from the long-lived SHV eruption , held at MVO, provides the best archive of material to examine the theories for variable toxicity offered above.
Elemental composition of cristobalite
Representative electron probe results for cristobalite and quartz phases
Sodium was also detectable in cristobalite, at levels up to 1.1 wt. % Na2O. The prismatic crystals contained 0 – 1.1 wt. % Na2O compared with 0.7 – 1.1 wt. % Na2O in the platy crystals. These data are also significantly different at the 5% level (p=0.0301). The platy cristobalite, in general, also contained slightly elevated levels of other major element oxides (e.g. K2O, Fe2O3, CaO and TiO2).
Cristobalite and associated phases
Observations of sectioned dome rock by Scanning Electron Microscopy (SEM) confirmed that cristobalite is intimately inter-grown with surrounding mineral phases and glass (Figure 1a). Additionally, devitrification crystallites of cristobalite are sub-micron sized, and are closely associated with glass and feldspar (composition confirmed by Horwell et al. ) (Figure 1b). It was also observed that, whilst whole cristobalite crystals in the dome rock are usually 20-50 μm diameter (i.e. within the ‘inhalable’ sub-100 μm fraction, but larger than the ‘thoracic’ (sub-10 μm) or ‘respirable’ (sub-4 μm) fractions), ‘fish-scale’ cracking divides the cristobalite into roughly sub-10 μm segments (as can be seen in Figure 1a and b).
Factors affecting cristobalite toxicity in Soufrière Hills ash
Electron microprobe analyses of in situ vapour-phase cristobalite crystals in dome rock confirmed that the Soufrière Hills cristobalite is not pure SiO2 (theory 1). The fact that Al has been shown to be the dominant substituted cation in cristobalite is critical for understanding its toxic potential.
The toxicological effects of cristobalite surface modification have not been previously studied, but it is known that the structure of cristobalite allows substitutions of Al for Si . The mechanism by which Al interacts with quartz is not well understood though (it does not have the open lattice structure of cristobalite or tridymite); however, in acidic conditions, Al is thought to insert into the quartz crystal lattice  at discrete points where defects enable this bonding to occur . Duffin et al.  treated laboratory standard DQ12 quartz surfaces with Al lactate which had the effect of inhibiting the production of cytokines from macrophages and epithelial cells. The release of reactive oxygen species, capable of causing mutations in the cells of the lung, a likely mechanism for the development of cancer , would also be affected if the silica surface were affected by Al. Analogously, the presence of Al in the cristobalite structure and, therefore, at discrete points at its surface, could have similar effects. To test the toxicity side of this structure-toxicity relationship, we are now synthesising cristobalite in the laboratory with controlled levels of Al and Na. The resulting powders will be exposed to alveolar macrophages and cytotoxic, metabolic and pro-inflammatory effects measured to test our theory that structural substitutions in cristobalite affect toxic response.
These structure substitutions may also control crystal morphology. The platy cristobalite contains significantly more Al and Na than prismatic crystals and, hence, at this level of substitution in the crystal structure, prismatic crystal formation may be inhibited. This leads to the possibility that differing cristobalite morphologies may also be variably pathogenic, depending on their Al content (i.e. platy crystals would be less toxic). However, Horwell et al.  did not observe solely platy crystals in these dome rock samples – they were always seen in association with prismatic and devitrification cristobalite (Table 1) - so it is unlikely that exposure would ever be restricted to the less-toxic variety.
A second mechanism for generation of cristobalite particles with heterogeneous surfaces is through dome-collapse fragmentation processes, generating particles which are mixed phases of cristobalite and other minerals (theory 2). Our results show this to be the case, as particles (in cross-section) are rarely observed to be solely comprised of cristobalite. These mixed-phase particles likely derive from: 1) vapour-phase crystals which were attached to (and will have nucleated from) groundmass or phenocrysts adjacent to the pore spaces, so we may expect that at least one surface of the vapour-phase cristobalite may have a 'rim' composed of other materials; or 2) break up of devitrified patches of glass where we would expect to see particles composed of feathery crystallites of cristobalite, plagioclase and a glassy matrix. These associated phases give an additional explanation as to why Horwell et al.  found that crystalline silica ash particles contain minor amounts of other elements (by SEM-EDS). It is also likely that many whole particles identified as plagioclase or glass by SEM-EDS e.g. by  would contain a crystalline silica component.
We did not observe evidence for any cristobalite surfaces being occluded by a thin coating of volcanic glass, clay, or an annealed amorphous layer (theory 3). In studies assessing the toxicity of coal dust, low-rank coals have been observed to contain crystalline silica coated with clays . The clays, and associated soluble extracts, in the coal mine dust are thought to inhibit the cellular reactivity of quartz . Fresh volcanic ash does not contain clay; however, old ash deposits, which may be quarried for volcanic aggregate, may well contain clays derived from chemical weathering of feldspar. Therefore, studies assessing the toxicity of crystalline silica-rich volcanic-derived quarry dust should assess whether silica occlusion is occurring.
When considering health, we can now say that, for the Soufrière Hills ash, there are two inherent factors which may act to reduce the toxicity of cristobalite: 1) the cristobalite is not pure SiO2 (theory 1); 2) heterogeneous RCS surfaces will be produced by fragmentation of devitrified areas and will consist of vapour-phase cristobalite and adhered fragments of groundmass (theory 2). All of these processes involve the introduction of Al to the RCS surface.
This evidence of surface modification and heterogeneity provides an explanation as to why the predicted toxicity is not observed in reality e.g. [28, 29] and also why SHV ash does not generate silica surface radicals (e.g., Si· and Si–O·) . However, there is also evidence here for the formation of reactive RCS volcanic particulate; the cracked, ‘fish-scale’ cristobalite provides weakened planes for the generation of inhalable sub-particles (< 15 μm diameter) of cristobalite on fragmentation and it appears that these surfaces remain exposed (i.e. no glass coating). The exposed crack intersections and twin planes at the surface of a crystal can be highly reactive  and may host transition metal ions, giving a possible source area for hydroxyl radical activity (HO·) . In addition, in areas of extensive devitrification, cristobalite can recrystallize to quartz, which may form up to 5 wt. % of dome rock in altered samples . The toxicity of quartz particles may be different to cristobalite and, therefore, a variably-altered volcanic dome may produce ash with a range of toxicities.
Considering external factors, the heterogeneity of volcanic ash, with the inevitable close association of cristobalite and Al-rich mineral particles (such as feldspar) in the lung could also contribute to impairment of RCS toxicity (theory 4). Diffusion of elements from dissolving/leaching particles to the RCS surface is a possibility on the lung surface but this is likely to be greatly increased within the acidic lysosomal environment following phagocytosis by macrophages, where several particles may be stored in close proximity. In industry, quartz is also extracted with other minerals (e.g. clays), for example, during quarrying and industrial processing of sedimentary rock. For coal-mine dust, it is believed that, where quartz levels are below 10 wt. %, the total mass dust exposure is the best correlate with coal workers’ pneumoconiosis (CWP) [51, 52]. However, above 10 wt. %, silicosis and rapid progression of CWP may occur . The lack of silica reactivity at low concentrations cannot only be due to occlusion of RCS surfaces by clays, as discussed above. When DQ12 quartz is pre-incubated with an extract of coal-mine dust, haemolysis drops from 90% in non-incubated DQ12 quartz to virtually zero in incubated samples, demonstrating that there is an external control, such as diffusion of ions to the quartz surface .
Determining if volcanic cristobalite toxicity is affected by external factors such as accompanying minerals is challenging as volcanic cristobalite cannot be isolated from the other minerals without affecting the surfaces of the particles (for example through heavy liquid separation or boiling the ash in phosphoric acid). Additionally, several minerals in volcanic ash are iron rich (e.g. amphiboles, pyroxenes and oxides) and Horwell et al. [21, 54] have shown that deleterious, iron-catalysed hydroxyl radicals can be generated by these minerals in Soufrière Hills ash, providing a possible separate mechanism for toxicity.
The variable hazard of RCS
Since IARC classified crystalline silica as a carcinogen, with the proviso that hazard was likely to vary depending on the characteristics of the dust , many authors have attempted to quantify the variability of the silica hazard. Studies have shown how cellular responses related to inflammation and fibrosis vary amongst different polymorphs and dust sources, and even amongst samples from the same source e.g. [48, 55]. Donaldson and Borm  have clarified that the surface reactivity of quartz is key, and that the ability of particles to induce oxidative stress could be modified by substances which affect its surface, including those derived from accompanying minerals. Therefore, toxicity could vary dramatically depending on the origin of the silica. Other authors have suggested that transient piezoelectric charges on freshly-fractured surfaces or the hydrophilicity of the surface may play a role in reactivity [57, 58].
In this study, we have investigated the mineralogical features which may modulate RCS toxicity. In the case of volcanic ash, we have shown evidence that the toxicity of RCS is likely affected by ‘inherent characteristics’ of the silica and hypothesized that ‘external factors’ may also act to hinder the reactivity of RCS. However, Fubini , postulated that the inherent characteristics and external factors may be active at different stages in disease development, or be involved in different disease pathways. There is also evidence that different disease mechanisms (e.g. inflammation and DNA damage) involve different properties of the silica particle [59, 60].
In occupational settings, RCS surfaces may be exposed and activated by grinding or milling [20, 61, 62], modified by the environment in which they are ground  and altered by heating (e.g., for fly ash and biogenic silicas) [63, 64]. These processes will variably alter their size, morphology, crystallinity, surface charges, hydrophilicity and external contaminants. However, the present investigation shows that the original mineralogy of the silica and its host material may also significantly influence its toxicity. Characterisation of these parameters can therefore shed light on the variable toxicity of respirable crystalline silica.
This study has shown that the surface properties and, therefore, toxicity of volcanic cristobalite particles are at least partly controlled by their geological origin. The results provide a compelling explanation for the anomalous toxicology data for cristobalite-rich volcanic ash and also highlight that inherent characteristics of industrial silica should be further studied. If RCS could be classified by its surface chemical composition and structure in relation to the factors that modify its hazard, then the single silica Occupational Exposure Limit (or Threshold Limit Value) currently adhered to across all industries could be modified to more closely represent the actual hazard of a particular RCS exposure.
Theory 1 (crystal composition) was addressed by electron microprobe (Cameca SX100 wavelength dispersive electron probe microanalyser (with PeakSight software) at the University of Cambridge, UK. See Additional file 1 for detailed methods) to determine the composition of vapour-phase ‘prismatic’ (n = 55) and ‘platy’ (n = 8) cristobalite crystals, devitrification quartz (n = 4) and magmatic quartz crystals (for comparison) (n = 2). Only data with totals of 100 ± 0.8% were used in the study, as is common practice for anhydrous rock studies. Dome rock was used to provide large, flat, polished areas of crystals for analysis, which are harder to obtain from ash particles. The statistical difference in the compositions of prismatic and platy cristobalite crystals was determined using Welch’s two-sample t-test.
To address theories 2 (heterogeneous surface) and 3 (annealed surface), we adopted two strategies: 1) imaging minerals in thin sections of dome rock to determine the association of cristobalite with other local minerals; 2) imaging individual cristobalite ash particles in cross section to distinguish a cristobalite ‘core’ and a potentially nano-scale ‘rim’ of other minerals or glass.
Imaging of dome rock was carried out on carbon coated (30 nm) thin sections in BSE mode, and combined with elemental analysis on a LEO 1455VP SEM with Oxford Instruments INCA Energy Dispersive (EDS) X-ray analysis system at the Natural History Museum, London (working distance = 14-15 mm, accelerating voltage = 20 kV).
Individual cristobalite crystals were thinned by focussed ion beam in a dual beam FIB-SEM (FEI Helios Nanolab at Durham University) to make them electron transparent . The rim (protected from the FIB by a platinum strip deposited by gas injection) was then analysed by TEM-EDS (JEOL 2100F FEG-TEM with Oxford INCAx-sight Si(Li) EDS software at 200 kV) over several 50 nm transects from the rim into the crystal. However, this time-intensive technique was not suitable for mass analysis of cristobalite crystals so just two cristobalite crystals were analyzed.
Cristobalite crystals were separated from ash sample MBA12/7/03 using heavy liquids and mounted in a resin block. The polished blocks were carbon coated (~30 nm) and analysed in the Hitachi SU-70 FEG-SEM at Durham University. High-resolution SEM allowed examination of hundreds of sections through cristobalite crystals with ~5 nm resolution. The Oxford Instruments EDS system (INCAx-act LN2-free analytical Silicon Drift Detector) allowed verification of particle elemental compositions e.g. cristobalite (almost entirely Si and O, with minor Al and Na), plagioclase feldspar (Si, O, Al, Na and Ca) and glass (Si, O and minor Al, K, Na, Ca and Fe), as defined by Horwell et al.  as well as compositional variations along ‘rim’ to ‘core’ transects (over a distance of 5-10 μm).
Respirable crystalline silica
International Agency for Research on Cancer
Scanning electron microscopy
Transmission electron microscopy
Focussed ion beam
Field emission gun
Energy dispersive X-ray spectroscopy
Pyroclastic density current
Soufrière Hills volcano, Montserrat.
The work of CH was supported by a NERC Postdoctoral Research Fellowship (Grant No. NE/C518081/2). JLB acknowledges a NERC CASE Studentship while at the University of Cambridge, UK (NER/S/A/2006/14107), and DD acknowledges a Moyes Foundation studentship. We are grateful to Sue Loughlin, Thomas Christopher and Montserrat Volcano Observatory staff for access to MVO collections and guidance in the field. We thank Chris Hayward, formerly of University of Cambridge, now at University of Edinburgh, UK, for assistance and training on the electron microprobe. Thanks to Budhika Mendis, GJ Russell Microscopy Facility, Durham University, for TEM work and Ed Llewellin (Durham University) for statistical expertise. Thanks also to Jeremy Skepper (University of Cambridge) for early development of particle sectioning techniques. We are grateful to Dr Peter Baxter (University of Cambridge) for reading a draft of the manuscript and to the three anonymous reviewers.
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