Figure 3

Controls for the cell-mediated cytotoxic potential of NK. (A) Representative dot plot of the NK-target cell mixture. Target cells were gated based on side scatter (SSC) properties and CFSE staining. (B-E) NK cell viability is not affected signifcantly by treatment DEP. Only cells gated for NK cells (based on SSC and negative CFSE staining) are shown. (F&G) The live-dead dye 7-AAD is not adsorbed by DEP. (F) 7-AAD pre-incubated with DEP for 15 min before staining a control sample of NK-target cell mixture after 4 hrs of incubation. (G) Control sample stained with 7-AAD normally after 4 hrs of incubation. (H&I) DEP do not kill target cells. (H) Target cells only stained with 7-AAD. (I) Target cells only incubated with DEP and stained with 7-AAD.