Figure 1

Interaction of 50 nm-FITC-SiO ₂ with NCI-H292. A. 3D reconstruction of a confocal analysis of the cells exposed to 50 nm-FITC-SiO₂ NPs at 5 μg/cm² for 24 h. Staining of the cells is as follows: Blue - DAPI-stained nuclei, Red – TRITC-phalloidin-stained actin filaments, Green – FITC-labelled SiO₂ NPs. Scale bar shows 10 μm. B. The same field of the confocal image shown in the Figure 1A presented as a projection of all images acquired in the stack. C. 3D reconstruction of x,z and y,z-slices of the corresponding regions on the image 1A. The insert shows one selected representative cell and D. Cells were exposed to different concentrations of NPs at indicated time points, followed by FCM analysis of median fluorescence intensity (MFI) of at least 10.000 cells. Results are represented as mean MFI value ± SD, n=3 of one out of 3 independent experiments. Data were analyzed by ANOVA, followed by Bonferroni post hoc test. * significantly different from previous time point, p < 0.05.