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Figure 11 | Particle and Fibre Toxicology

Figure 11

From: Deciphering the mechanisms of cellular uptake of engineered nanoparticles by accurate evaluation of internalization using imaging flow cytometry

Figure 11

Internalization of TiO 2 NPs by NCI-H292 cells analyzed by imaging flow cytometry. A. Representative images captured by Amnis ImageStreamX Flow Cytometer of cells treated with neutral, positively or negatively charged TiO2 NPs for 4 h at 20 and 40 μg/cm2. First column shows cells in the brightfield (BF), second shows the mask eroded for 3 μm, third column shows the signal of the side scatter (SS) and forth column shows images of the brightfield merged with the side scatter signal (BF/SS). B. Intensity of the side scatter signal inside the mask eroded for 3 μm. Results are expressed as mean value ± SD of three independent experiments analyzing at least 500 cells. Data were analyzed by ANOVA followed by Bonferroni post hoc test. * significantly different from control, p < 0.05.

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Particle and Fibre Toxicology

ISSN: 1743-8977

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