Figure 9
Effect of siRNA induced gene silencing of clathrin heavy chain on SiO 2 NP uptake. Cells were treated with siRNA-control or siRNA-clathrin heavy chain for 72 h before treating with A.: 50 nm-FITC-SiO2 NPs for 3.5 h. Quantification of the internalization was performed by flow cytometry after addition of TB. Results are expressed as percentage of NP uptake compared to uptake in the siRNA-control treated cells, mean percentage ± SD, n = 9 B.: 15 μg/cm2 of 100 nm-Por-SiO2 NPs for 3.5 h. Analysis of the internalization was performed by imaging flow cytometry using a mask eroded for 3 μm. Results are expressed as mean Internalization score ± SD of three independent experiments.