Internalization and cytotoxicity of graphene oxide and carboxyl graphene nanoplatelets in the human hepatocellular carcinoma cell line Hep G2

Table 2 Hydrodynamic size distribution in CXYG suspensions as function of concentration and incubation time

CYXG in MEM+		Average hydrodynamic diameter						Relative intensity
Time	Conc.	Peak 1		Peak 2	Peak 3	Peak 4	Peak 5	Peak 1	Peak 2	Peak 3	Peak 4	Peak 5	PdI
[hrs]	[μg/ml]	d. ± SD [nm]		d. ± SD [nm]	d. ± SD [nm]	d. ± SD [nm]	d. ± SD [nm]	[%]	[%]	[%]	[%]	[%]
0	32	-	-	-	349.5 ± 42.6	1805.6 ± 616.8	4827.3 ± 497.5	0.0	0.0	15.1	74.1	10.7	0.7
0	16	5.4	n = 1	52.5 ± 9.8	342.5 ± 74.5	2333.8 ± 380.9	4852.5 ± 29.0	0.2	1.4	18.7	74.4	4.3	0.9
0	8	10.2	± 1.9	35.1 ± 9.2	116.2 ± 33.3	796.8 ± 69.3	5449.0 n = 1	9.1	3.6	12.2	74.8	0.4	0.8
0	4	9.7	± 1.4	41.2 ± 9.3	153.1 ± 8.2	821.7 ± 176.3	5516.0 n = 1	17.9	13.2	4.6	64.0	0.3	0.7
48	32	-	-	-	259.3 ± 33.1	1451.3 ± 373.2	5225.5 ± 121.0	0.0	0.0	6.9	89.3	3.7	0.5
48	16	9.7	± 2.1	20.4 ± 1.3	316.2 ± 22.3	1741.7 ± 409.2	4766.0 ± 37.6	2.4	1.3	4.8	87.2	4.4	0.7
48	8	9.0	± 1.4	35.7 ± 8.1	-	896.6 ± 117.5	5134.0 n = 1	9.2	7.5	0.0	83.0	0.3	0.8
48	4	9.2	± 2.3	37.2 ± 11.2	78.4 ± 24.8	735.0 ± 59.0	-	16.0	13.7	0.8	69.6	0.0	0.6
120	32	-	-	69.0 n = 1	283.2 ±164.0	1527.2 ± 483.5	5216.3 ± 286.8	0.0	0.1	14.3	87.7	5.4	0.6
120	16	11.8	± 1.8	-	87.3 ± 21.9	934.3 ± 117.7	-	3.3	0.0	7.1	88.4	0.0	0.6
120	8	9.8	± 2.1	44.4 ± 18.6	-	820.8 ± 40.8	5549.0 n = 1	10.8	7.6	0.0	81.4	0.3	1.0
120	4	10.3	± 0.9	-	107.2 ± 39.0	451.2 ± 83.8	5184.0 ± 116.8	3.2	0.0	14.7	79.9	2.1	0.6
blank		-	-	9.6 ± 0.4	62.4 ± 2.5	-	4268.5 ± 129.9	0.0	49.6	46.9	0.0	3.5	0.5

DLS analysis was performed on serial dilutions of a CXYG suspension (32 μg/ml) prepared in culture medium supplemented with 10% FBS. The samples were analyzed directly after preparation as well as after incubation at 37°C for 48 and 120 h, respectively. The average hydrodynamic size is represented as mean diameter (d.) ± standard deviation (SD) in nm. Data are from six consecutively performed measurements. The relative intensities of the size peaks are indicated in [%], respectively. The polydispersity index (PdI) of the analyzed samples is shown in the last column. “n = 1” indicates that no SD could be calculated since the peak was detected in only one of the six performed measurements. The minus (“-“) indicates that no peak could be identified at the respective dilution stage.

ISSN: 1743-8977