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Figure 3 | Particle and Fibre Toxicology

Figure 3

From: An improved 3D tetraculture system mimicking the cellular organisation at the alveolar barrier to study the potential toxic effects of particles on the lung

Figure 3

Z- stack image series to analyse the distribution of THP- 1 macrophages and HMC- 1 in the tetraculture system present in the apical compartment of the insert. The distribution of A549, differentiated THP-1, HMC-1 and EA.hy 926 cells in the tetraculture was analysed via CLSM. Cellular membranes are stained with cell mask deep red dye (red) and nuclei are stained with DAPI (blue); Macrophage-like cells are counterstained with an anti-CD11b-antibody. A: X–y projection with the respective side views. B: 3D reconstruction of the tetraculture based on the results of the z-stack from A. THP-1 (green arrows) and HMC-1 (blue arrows) cells are found on top of the epithelial cells. EA.hy 926 cells were not considered in the 3D reconstruction.

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Particle and Fibre Toxicology

ISSN: 1743-8977

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