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Figure 6 | Particle and Fibre Toxicology

Figure 6

From: An improved 3D tetraculture system mimicking the cellular organisation at the alveolar barrier to study the potential toxic effects of particles on the lung

Figure 6

DCFH -DA assay to analyse the behaviour of the different cultures in response to oxidative stress. Cultures were preloaded with DCFH-DA dye for 45 min and subsequently exposed to 20 mM AAPH in medium for 2 h. The oxidation of DCFH-DA was measured as an augmentation of green fluorescence. The increase in ROS production was compared to cells that were treated with medium without AAPH. Data represents the mean of at least four independent transwell inserts ± SEM. Groups that are sharing the same letters are not significantly different (P > 0.05).

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