PRDX6 mRNA and protein expression. RT-PCR: (A) Basal PRDX6 mRNA was similarly expressed between ages and compartments. (B) Neonatal parenchymal PRDX6 mRNA was increased 48 hours post exposure. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), ‡ significantly different compared to FA in the same compartment. (C) Adult PRDX6 mRNA was unaffected by PFP. (D) Scan of PRDX6 and Actin Western blots. (E) Neonatal PRDX6 protein expression was unchanged by PFPs. (F) Adult PRDX6 protein expression trended upwards at PFP48. (G-J) Immunohistochemical localization of PRDX6 protein in lung tissue (n=6 rats/group). No substantial age and compartment differences were observed post exposure. Scale bar is 50 μm.