Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2 antioxidants differently in neonatal and adult rat lungs

Table 4 Summary of PFP induced antioxidant expression

	Assay	Lung compartment	Neonates	Adults
HMOX1	RT-PCR	Airway	NC	NC
		Parenchyma	+	+
	WB	Whole Lung	NC	+
	IHC	Airway	+	+
		Parenchyma	NC	+
SOD1	RT-PCR	Airway	NC	+
		Parenchyma	-	NC
	WB	Whole Lung	NC	+
	IHC	Airway	NC	+
		Parenchyma	NC	+
SOD2	RT-PCR	Airway	NC	NC
		Parenchyma	NC	-
	WB	Whole Lung	NC	NC
	IHC	Airway	NC	NC
		Parenchyma	NC	NC
CAT	RT-PCR	Airway	NC	NC
		Parenchyma	NC	-
	WB	Whole Lung	NC	NC
	IHC	Airway	NC	+
		Parenchyma	NC	+
PRDX6	RT-PCR	Airway	NC	NC
		Parenchyma	+	NC
	WB	Whole Lung	NC	NC
	IHC	Airway	NC	NC
		Parenchyma	NC	NC

ISSN: 1743-8977