Figure 4

SAEC exposure to MWCNT increases the expression of secreted inflammatory mediators in coculture. SAEC and HMVEC were grown in the apical and basolateral chambers, respectively, of a co-culture system and SAEC exposed to 1.2 μg/ml MWCNT for 24 hours. Apical and basolateral media were collected and assayed by ELISA for VEGFA, sICAM1, and sVCAM1 protein expression. All values given are the mean ± standard error. VEGFA levels (A) increased from 87.15 ± 6.58 pg/ml to 114.96 ± 14.89 pg/ml in the basolateral chamber and 313.45 ± 27.85 pg/ml to 378.38 ± 21.89 pg/ml in the apical chamber. sICAM1 levels (B) increased from 107.99 ± 6.14 pg/ml to 123.76 ± 3.00 pg/ml in the basolateral chamber and 44.13 ± 3.04 pg/ml to 55.56 ± 2.63 pg/ml in the apical chamber. sVCAM1 levels (C) increased from 38.77 ± 7.52 pg/ml to 46.07 ± 11.07 pg/ml in the basolateral chamber and 43.69 ± 6.38 pg/ml to 85.29 ± 11.64 pg/ml in the apical chamber. * p < 0.05 above control.