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Figure 3 | Particle and Fibre Toxicology

Figure 3

From: Asbestos and erionite prime and activate the NLRP3 inflammasome that stimulates autocrine cytokine release in human mesothelial cells

Figure 3

NLRP3 inhibition attenuates IL-1β and HMGB1 release from HMC. (A) Inhibition of NLRP3 using siNLRP3 in LP9 cells significantly inhibits siNLRP3 mRNA levels (* = p ≤ 0.05 as compared to siCon group) and (B) Inhibition of NLRP3 using siNLRP3 in LP9 cells significantly attenuated NLRP3 protein levels at 78 h. (C) Production of mature IL-1β, (* = p ≤ 0.05 as compared to untreated siCon cells; † = p ≤ 0.05 as compared to siCon asbestos-exposed group). (D) Crocidolite asbestos increases HMGB1 release that is blocked in siNLRP3-transfected cells at 24 h (N = 3 samples/group). * = significantly different (p ≤ 0.05) when compared to siCon group; † = significantly different (p ≤ 0.05) when compared to siCon asbestos-exposed group. Priming effect of TNFα on IL-1β was blocked by siNLRP3 at 48 h (E) (N = 2, 4 for asbestos alone groups, * = significantly different (p ≤ 0.05) from siCon untreated (0) group).

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Particle and Fibre Toxicology

ISSN: 1743-8977

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