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Table 5 RT-PCR validation of selected genes from microarray data

From: RETRACTED ARTICLE: Intragastric exposure to titanium dioxide nanoparticles induced nephrotoxicity in mice, assessed by physiological and gene expression modifications

Function

Gene

ΔΔCt

Fold

Microarray

Apoptosis

Apaf1

0.516327

0.699149555

0.5535156

 

Ngfrap1

0.15624

0.897360758

0.6713378

 

Cycs

0.34444

0.787613639

0.7045653

 

Tnfrsf12a

0.04954

0.966244365

0.655954

 

Bcl2l1

-1.131909

2.191485298

1.663064

 

Birc5

3.213009

0.107841995

0.10103

 

Fn1

0.470814

0.721557364

0.4940369

 

Ccl19

-2.490308

5.618978967

4.313047

 

Ccl21a

-1.826821

3.547545038

2.850085

Immune/Inflammatory response

Bmp6

-1.291201

2.447317025

40.56928

 

Cd55

0.54583

0.684997202

0.3824578

 

Cd74

-1.327117

2.509007877

1.832029

 

Cfd

-1.861094

3.632830363

2.601335

 

Cfi

1.259912

0.417569429

0.3720603

 

Cxcl12

-1.913449

3.76708608

2.043431

 

C3

-1.580409

2.990546189

1.886813

 

Cd34

0.282201

0.822335491

0.4588617

 

Bcl6

-2.069258

4.196707749

3.073497

Oxidative stress

Cygb

-1.439087

2.711492162

1.646112

 

Gpx7

0.585512

0.666412792

0.3389097

 

Psmb5

0.057543

0.960899198

0.6914788

Cell cycle

Klf1

-1.646997

3.131810673

2.100916

 

Bub1b

3.216187

0.1076047

0.2573011

 

Txnip

-1.615053

3.063228525

1.648611

Signal transduction

Egr1

0.348792

0.785241322

0.5866718

 

Nid1

0.918823

0.528940372

0.3838886

Biological process

Odc1

1.576015

0.335407069

0.3957967

 

Serpinalb

-2.035634

4.100028676

3.299695