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Figure 5 | Particle and Fibre Toxicology

Figure 5

From: Iron oxide nanoparticles suppress the production of IL-1beta via the secretory lysosomal pathway in murine microglial cells

Figure 5

IONPs increased the alkalinity and permeability of lysosomes in LPS-stimulated microglia. Primary microglial cells (4 × 105 cells/mL) were either left untreated (naïve; NA), or pretreated with IONPs (1–50 μg Fe/mL) for 30 min followed by stimulation with LPS (100 ng/mL) for 24 h. (A and B) The acidity of lysosomes was evaluated by flow cytometry with lysosensor Green DND-189 staining. (C and D) The permeability of microglial cells were measured by flow cytometry with acridine orange staining. (B and D) Representative histograms of lysosensor and acridine orange in cells pretreated with IONPs (50 μg Fe/mL) and stimulated with LPS were shown. Data are expressed as the mean ± SE of triplicate cultures. Results are a representative of three independent experiments. * p < 0.05 compared to the LPS group. MFI, mean fluorescence intensity.

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