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Table 3 BALF cytology and estimation of NP endocytosis by macrophages following inhalation exposure

From: Rat pulmonary responses to inhaled nano-TiO2: effect of primary particle size and agglomeration state

Experimental groups
Cell type Control 5 nm SA 5 nm LA 10-30 nm SA 10-30 nm LA 50 nm SA 50 nm LA
Total cells a 2.36±0.46 4.32±0.27* 4.02±0.63 4.41±0.40* 5.38±0.45** 3.01±0.36 4.82±0.36**
Lymphocytes b 4.16±0.60 2.15±0.37 8.08±1.97 4.57±0.82 7.02±2.67 2.85±0.49 4.34±1.37
Neutrophils c 0.23±0.05 1.18±0.19 1.18±0.34 1.26±0.16 1.47±0.41* 0.84±0.18 1.78±0.22**
Macrophages a 2.30±0.45 4.18±0.27* 3.82±0.59 4.24±0.39* 5.16±0.42** 2.90±0.35 4.60±0.36**
Estimation of endocytosis (%) d --- 53±3.8ŧ 78±1.3 53±1.9ŧ 78±2.9 65±1.9ŧ 83±1.3
  1. a number of cells x 106 ± standard error on the mean (SEM); b number of cells x 104 ± SEM; c number of cells x 105 ± SEM; d estimation of the extend of NP phagocytosis by macrophages. Mean values (n = 6 rats per group) significantly different from controls (ANOVA followed by a Tukey’s test) *p<0.05, **p<0.01, ŧp<0.05 significantly different from LA aerosol of the same primary NP size.