Figure 9
Confocal evaluation of tissue distribution of QD. Confocal micrographs of lungs (green) that have been cryo-preserved, sectioned, and imaged at 63x, 2 hours (A-C) and 1 day (D-F) after intratracheal instillation with 12.5 μg of QD-NH2, QD-COOH, or PBS (control): (A) control – 2 hours post-exposure, (B) QD-NH2 - 2 hours post-exposure, (C) QD-COOH – 2 hours post-exposure, (D) control – 1 day post-exposure, (E) QD-NH2 - 1 day post-exposure, and (F) QD-COOH – 1 day post-exposure. Lung tissue auto-fluoresces green and QD are yellow/red. * indicates QD-containing AM, white arrows indicate QD in tissue.