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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: Towards predicting the lung fibrogenic activity of nanomaterials: experimental validation of an in vitro fibroblast proliferation assay

Figure 2

MWCNT stimulate fibroblast proliferation in vitro and induce lung fibrosis in a dose-dependent manner. (A) In vitro effect of MWCNT on primary murine lung fibroblast proliferation assessed by cell counting. Fibroblasts cultured from C57BL/6 mouse lungs were treated with different doses (μg/cm2 culture well) of MWCNT (NM400 and NM402, final FBS concentration 0.2%) for 24 h. PDGF-BB (50 ng/ml) was used as a positive control. Results are expressed relative to non-treated cell number (Ctl). * p < 0.05, ** p < 0.01, *** p < 0.001 vs non-treated cells (n = 3, t-test or Dunnet multiple comparison test as appropriate). (B) In vivo lung fibrotic response to MWCNT (hydroxyproline lung content) assessed 2 months after pharyngeal administration in mice with different doses of MWCNT (NM400 and NM402), or Min-U-Sil (SiO2) as positive control. * p < 0.05, ** p < 0.01, *** p < 0.001 vs non-treated cells (n = 5-7, t-test or Dunnet multiple comparison test as appropriate).

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