Figure 4From: Towards predicting the lung fibrogenic activity of nanomaterials: experimental validation of an in vitro fibroblast proliferation assay In vitro effect of MWCNT on proliferation of several fibroblast types assessed by cell counting. Primary murine lung fibroblasts and human and mouse fibroblast cell lines (HFL-1, BALB/3T3 and MLg) were treated for 24 h with different doses of NM400 dispersed in 1.4 mg BSA /ml (final concentration 0.14 mg BSA /ml). PDGF-BB (50 ng/ml) was used as a positive control. Results are expressed relative to non-treated cell number (Ctl). * p < 0.05, ** p < 0.01, *** p < 0.001 vs non-treated cells (n = 3, t-test or Dunnet multiple comparison test as appropriate).Back to article page