Figure 5

In vitro effect of MWCNT on MLg fibroblast proliferation assessed by different assays. Fibroblasts were treated 24 h with different doses of NM400 dispersed in 1.4 mg BSA /ml (final concentration 0.14 mg BSA /ml). (A) Cell counting, (B) WST-1 assay and (C, D) propidium iodide staining (C) % cells in S and G2/M phases and (D) histogram plot event counts vs PI were used for in vitro assays. PDGF-BB (30 ng/ml) was used as a positive control. Results are expressed relative to cell number, WST1 activity or % cells in S/G2/M in non-treated cells (Ctl). * p < 0.05, ** p < 0.01, *** p < 0.001 vs non-treated cells (n = 3-4, t-test or Dunnet multiple comparison test as appropriate).