Figure 10

Example of how MWCNT retention is determined in an unstained lung section. A) Low resolution digitized image, composed of auto-fluorescence (tissue) and light loss (MWCNT), of lung section from N/S-O MWCNT-exposed mouse. Blue box indicates the scanning area. B) High resolution digitized image, composed of auto-fluorescence (tissue) and light loss (MWCNT), of scanning area from Figure 10A. C) Gated scatter-gram with total scan areas collected where the purple area indicates positive phantom contours for particle deposition. D) Low resolution digitized image, composed of auto-fluorescence (tissue) and light loss (MWCNT), of lung section from N/L-O MWCNT-exposed mouse. Blue box indicates the scanning area. E) High resolution digitized image, composed of auto-fluorescence (tissue) and light loss (MWCNT), of scanning area from Figure 10C. F) Gated scatter-gram with total scan areas collected where the purple area indicates positive phantom contours for particle deposition.