Serum from C57Bl6 mice exposed to MVE increases BBB permeability in mouse BBB co - culture. BBB permeability was quantified by measuring sucrose permeability across the apical membrane of the BBB co-culture. Serum (500 μl) from C57Bl6 mice exposed to either: filtered air (FA) or 100 PM μg/m3 of mixed vehicular emission (MVE) for 6 hr/day, for 30 days was applied to the BBB co-culture on the apical compartment (24 hrs). Data are sucrose apparent permeability coefficients (Papp). As determined by scintillation counting (amount of tracer that passed through the apical compartment). Each data point represents the mean ± SE of n = 3 animals (three BEC monolayers each). *p < 0.050 compared to FA control.