Figure 7From: Exposure to vehicle emissions results in altered blood brain barrier permeability and expression of matrix metalloproteinases and tight junction proteins in mice Expression of tight junction protein occludin, in cerebral microvessels (frontal cortex) from Apo E−/− mice exposed to mixed vehicular emission or filtered air. Double immunofluorescence of occludin (green fluorescence: A, D) and vonWillebrand factor (vWF) (red fluorescence: B, E) in cerebral microvessels (frontal cortex) from Apo E−/− mice exposed to 100 μgPM/m3 of mixed vehicular emission (MVE: A – C) or filtered air controls (FA: D - F) for 6 hr/day, for 30 days. Colocalized expression of occludin and vWF in microvascular endothelial cells is shown in panels C and F, indicated by yellow fluorescence. Colocalization was determined by quantifying total fluorescence of overlayed signals from minimum of three slides, two sections each, three regions from each section (n = 4–5 per group), which is represented by the graph shown in panel G. Arrows indicate expression of occludin (D) and endothelial-cell specific occludin expression (F) in the cerebral microvasculature of FA animals, which is measurably decreased in the microvessels from MVE-exposed animals (A and C, respectively). Scale bar = 10 μm; 100× magnification. Control slides with no primary antibody were also done (not shown) to confirm specific binding. *p < 0.050 compared to FA control.Back to article page