Figure 2From: Cytotoxicity and genotoxicity of nano - and microparticulate copper oxide: role of solubility and intracellular bioavailabilityDissolution of CuO NP and CuO MP in different media. CuO NP and CuO MP were incubated in (A) H2O or AAF for 24, 96 or 168 h at 37°C, (B) in DMEM/FCS for 2, 4, 8, 16 or 24 h or in DMEM without FCS for 24 h under cell culture conditions (37°C, 5% CO2, 100% humidity) or (C) in ALF for 0.5, 1, 2, 4, 24, 96 or 168 h at 37°C. Remaining particles were removed from the solution by multiple centrifugation as described in Materials and methods. The copper content was quantified by GF-AAS. Shown are mean values of 3 independent determinations + SD. 100% total copper refer to 39.95 mg/L in case of 50 μg/mL CuO. Please note the different scales of the Y-axis.Back to article page