Figure 7
Inhibition of H 2 O 2 -induced poly(ADP-ribosyl)ation by CuO NP, CuO MP or CuCl 2 . Logarithmically growing HeLa S3 cells were preincubated with the respective copper compound for 24 h and treated with 100 μM H2O2 for 5 minutes in the continued presence of CuO NP, CuO MP or CuCl2. Afterwards, cells were washed, fixed and processed as described in Materials and methods. The data represent mean values of at least 6 determinations based on the evaluation of at least 50 cells + 95% confidence intervall (CI). Statistically significant different from control: *p < 0.05, **p < 0.01, ***p < 0.001 as determined by unifactorial analyis of variance (ANOVA) followed by Dunnett T/T3 test in case of homogeneous (CuO NP, CuCl2)/ inhomogeneous (CuO MP) variances. Significant difference between 252 μM CuCl2 and 20 μg/ml CuO NP as determined by Students’ t-test: *p < 0.05. 1 μg/mL are equal to 0.2 μg/cm2 and 20 μg/mL CuO are equal to 252 μM Cu2+ in case of complete dissolution.