Skip to main content
Figure 8 | Particle and Fibre Toxicology

Figure 8

From: Cytotoxicity and genotoxicity of nano - and microparticulate copper oxide: role of solubility and intracellular bioavailability

Figure 8

Copper bioavailability and intracellular distribution after incubation with CuO NP, CuO MP or CuCl 2 in A549 cells. Logarithmically growing A549 cells were incubated for 24 h with CuO NP, CuO MP or CuCl2. Subsequently, (A) nuclear and (B) cytoplasmic protein fractions were isolated and the copper content was quantified by ICP-MS as described in Materials and methods. The data represent mean values of at least 3 independent experiments + SD. Statistically significant different from control: *p < 0.05, **p < 0.01 as determined by unifactorial analysis of variance (ANOVA) and Dunnett’s T3 test. 1 μg/mL are equal to 0.2 μg/cm2 and 20 μg/mL CuO are equal to 252 μM Cu2+ in case of complete dissolution.

Back to article page