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Figure 3 | Particle and Fibre Toxicology

Figure 3

From: Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia

Figure 3

NP impact on cell viability and epithelial integrity. Cell viability probed with the MTT assay (A) or trypan blue exclusion assay (B) after 48 h of exposure to TiO2-NP concentrations ranging from 0 to 200 μg/mL. Results are expressed as average of eight replicates (MTT) or three replicates (trypan blue) ± standard deviation; they were considered statistically significant (*) when p < 0.05. Caco: Caco-2, HT29: HT29-MTX. Assessment of apoptosis and necrosis in control epithelia or epithelia exposed for 48 h to 50 μg/mL of TiO2-NPs (C): cells were stained with acridin orange/ethidium bromide; viable cells stain green, apoptotic cells stain orange and necrotic cells stain red.

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