Figure 7

BAL cytokines and lipid mediators after CNP instillation. A) Heat map of cytokines. Black boxes indicate lowest and bright red highest detected levels for each cytokine (according to Table 3). Cytokines have been sorted by the response pattern. IL-1a, IL-6, and CXCL1 showed highest levels at day 1 after CNP exposure, CCL5, OPN, and IL-12p40 at day 3. Overall elevated cytokine levels were observed in Cftr mutant (−/−) as compared to WT (+/+) mice. B) 15-HETE. Increase over time after instillation treatment in WT mice, but independently of the CNP exposure; 15-HETE was generally reduced in Cftr mutant mice (hatched bars). *: significant difference to WT animals, p < 0.05. CC: cage control (untreated animals); Sham: instillation of 50 μL pyrogene-free distilled water.