Isolation and characterization of CSC-like cells from SWCNT-exposed lung epithelial cells. (A) SP and non-SP (NSP) FACS sorting profiles of malignant transformed BSW and lung cancer H460 cells. Due to their highly dynamic nature of CSC, cells were freshly isolated prior to each experiment. (B) Light scattering properties of SP (red) and NSP (blue) cells derived from BSW and H460 cells as evaluated by flow cytometry. (C, D) Analysis of colony formation (C) and tumor spheres (D) in SP and NSP cells derived from BSW and H460 cells after two weeks of culture.