Figure 2
Crocidolite asbestos exposure causes oxidation of Trx1 in human mesothelial cells. (A) LP9/h-TERT cells were exposed to crocidolite asbestos at 75 × 106 μm2/cm2 for 8 and 24 h and 40 μg of protein was run on a 15% native gel and a redox Western blot analysis was performed as described in the Methods section on the immobilized proteins. (B) Quantitation of the blot in (A). (C) Cells were exposed to two concentrations of crocidolite asbestos (15 × 106 μm2/cm2 and 75 × 106 μm2/cm2), as well as chrysotile asbestos (75 × 106 μm2/cm2) and a redox Western analysis was performed on the lysates (*p < 0.05 compared to null controls (n = 2 per group).