Figure 6

CCL2 mRNA and protein levels in the lungs of mice after NiNP exposure. A) CCL2 mRNA expression was measured by qRT-PCR in whole lung tissue at 1 and 21 days post exposure while B) CCL2 protein in BALF was analyzed by ELISA after 1 or 21 days of initial exposure (nd, not detectable). *p < 0.05, **p < 0.01, ***p < 0.001 as compared to the control group of the same genotype or as indicated. Data are the mean values ± SEM (n = 5–7 animals/group).