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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: The influence of hydrogen peroxide and histamine on lung permeability and translocation of iridium nanoparticles in the isolated perfused rat lung

Figure 2

Experimental protocol for isolated perfused rat lungs. All perfusions were done under negative pressure ventilation. Following a 15 minute period of equilibration, during which the lungs were already ventilated and perfused, the experiment started by the inhalation of freshly produced 192Ir-UFP from the aerosol line. Intratracheal instillation of 99mTc-DTPA or other instillations were performed at this starting time point. The perfusate was collected continuously and sampled at 15-min time intervals. The following treatments were investigated, group 1: control group, only 192Ir-UFP inhalation for 120 min; group 2: instillation of 50–100 μL 99mTc-DTPA, 500 μl H2O2 bolus (0.5 mM), 192Ir-UFP aerosol inhalation for 120 min; group 3: instillation of 50–100 μL 99mTc-DTPA, histamine continuously pefrused during the next 2 hours at concentration 10 μM, 192Ir-UFP aerosol inhalation for 120 min; group 4: instillation of 50–100 μL 99mTc-DTPA and 500 μl histamine bolus instillation at a concentration of 10 mM, 192Ir-UFP aerosol inhalation for 120 min; group 5: instillation of 50–100 μL 99mTc-DTPA. For each group 3–4 animals were used.

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