A method for exposing rodents to resuspended particles using whole-body plethysmography

Table 3 Daily and average HP12 mass (mg) collected on gravimetric filters

	Chamber 1	Chamber 2	Chamber 3	Chamber 4	Chamber 5	Chamber 6	Average	RSD^a
Phase 1
Day 0	0.620	0.486	1.022	0.526	0.310	0.551	0.586	40.6%
Day 1	0.867	0.656	0.848	0.617	0.575	0.447	0.668	24.3%
Day 2	0.973	0.634	0.656	0.765	0.860	0.763	0.775	16.4%
Day 3	1.497	0.686	0.919	0.856	1.158	1.285	1.067	28.2%
Average	0.989	0.616	0.861	0.691	0.726	0.761	0.774	--
RSD	37.3%	14.4%	17.9%	21.4%	50.3%	49.0%	--	35.5%
Phase 2
Day 0	2.473	1.201	1.701	1.427	1.568	1.456	1.638	27.0%
Day 1	1.706	1.671	1.601	2.111	1.986	1.175	1.708	19.2%
Day 2	0.926	1.513	1.902	1.627	1.849	--^b	1.563	25.0%
Day 3	1.496	2.062	1.832	1.529	1.244	2.135	1.716	20.4%
Average	1.650	1.612	1.759	1.674	1.662	1.589	1.657	--
RSD	38.8%	22.2%	7.63%	18.1%	19.8%	31.1%	--	21.6%
Phase 3
Day 0	1.214	2.677	5.425	2.971	2.283	2.634	2.867	48.6%
Day 1	2.715	1.973	4.406	2.997	1.141	2.740	2.662	41.0%
Day 2	1.287	1.902	3.038	1.941	1.152	2.287	1.935	35.6%
Day 3	1.457	4.739	1.602	2.050	2.279	1.510	2.273	55.1%
Average	1.668	2.823	3.618	2.490	1.714	2.293	2.434	--
RSD	42.3%	46.9%	45.9%	23.0%	38.2%	24.3%	--	46.1%
Phase 4
Day 0	1.780	1.783	2.661	3.038	1.762	3.673	2.450	32.9%

^aRSD = relative standard deviation
^bParticle mass was not obtained for this day.
Filters were attached in-line to the exhaust of individual whole-body plethysmograph chambers (see Figure 2). Accumulated mass was obtained for single 6-hr exposures.

ISSN: 1743-8977