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Table 1 Two protocols for conventional and immuno TEM preparations routinely used in our lab.

From: Visualization and quantitative analysis of nanoparticles in the respiratory tract by transmission electron microscopy

  cTEM Immuno TEM
Fixative 1.5% GA, 1.5% PFA in 0.15 M Hepes buffer 0.1% GA, 4% PFA in 0.2 M Hepes buffer
Postfixative 1% osmium tetroxide in sodium cacodylate buffer ---
En-bloc staining Half-saturated aqueous uranyl acetate 0.5% uranyl acetate in methanol (at -90°C)
Dehydration Ascending acetone series Methanol (at -90°C)
Embedding medium Araldite (at 60°C) Lowicryl HM 20 (at -45°C under UV light)
  1. Note. In this table two different protocols of specimen preparation for cTEM and immuno TEM, respectively, are given. Detailed descriptions of the protocols are given in Fehrenbach and Ochs [51]. Abbreviations: GA = glutaraldehyde, PFA = paraformaldehyde.