Table 3 Cell culture methods.
Cells | BEAS-2B | BEAS-2B | NHBE | A549 |
|---|---|---|---|---|
Supplier | US EPA | US EPA | Cambrex | ATCC |
Culture Media | KGM | LHC-9 | BEGM | DMEM/F12 10% FBS |
Supplier | Clonetics CC-3001 with CC-3111 kit | Biosource | Clonetics | ATCC |
Precoat on culture surface | No | No | Collagen/fibronectin | No |
Seeding in treatment wells | 35,000/cm2 | 35,000/cm2 | 2500/cm2 | 20,000/cm2 |
Seeding to PM treatment | 3 days | 3 days | 7 days | 3 days |
Passage Number | 75–85 | 75–85 | 5–7 | 80–82 |