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Table 3 Cell culture methods.

From: Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts

Cells BEAS-2B BEAS-2B NHBE A549
Supplier US EPA US EPA Cambrex ATCC
Culture Media KGM LHC-9 BEGM DMEM/F12 10% FBS
Supplier Clonetics CC-3001 with CC-3111 kit Biosource Clonetics ATCC
Precoat on culture surface No No Collagen/fibronectin No
Seeding in treatment wells 35,000/cm2 35,000/cm2 2500/cm2 20,000/cm2
Seeding to PM treatment 3 days 3 days 7 days 3 days
Passage Number 75–85 75–85 5–7 80–82
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