Figure 13
From: Optimized dispersion of nanoparticles for biological in vitro and in vivo studies
Preparation steps of nanoparticle dispersion. 1. Sonicate the nanoparticles in distilled water (power consumption: 7 W, 1 mL dispersion, 60 sec sonication, = 4.2 × 105 kJ/m3). 2. Add stabilizer (1.5 mg/ml HSA for dispersions with less than 0.2 mg/ml nanoparticle concentration or serum with a similar albumin concentration). 3. Add PBS to achieve physiological buffer and salt concentration.