Figure 2
From: Optimized dispersion of nanoparticles for biological in vitro and in vivo studies
Role of the sequence of preparation steps. TiO2 (rutile) dispersed in distilled water at a concentration of 0.02 mg/ml was sonicated with 4.2 × 105 kJ/m3 specific energy (US) or not sonicated (no US). Tween 80 0.1% (T), HSA 1.5 mg/ml (A) or distilled water (W) was given to the dispersion before (disp, PBS) or after (PBS, disp) addition of concentrated PBS. The average hydrodynamic diameter of the particles was measured (n = 4; *, p < 0.05 vs. dispersion in distilled water (W) without sonication (no US), #, p < 0.05 vs. dispersion in distilled water (W) with sonication (US), § p < 0.05).