Exposure to ultrafine carbon particles at levels below detectable pulmonary inflammation affects cardiovascular performance in spontaneously hypertensive rats

Table 3 Target sequences of the labelled probes used for detection of genes following amplification of cDNA using real time PCR

Gene Name	Gene Symbol*	Target Probe Sequence**
β-Actin	Actb	CTTCCTGGGTATGGAATCCTGTGGC
Hypoxanthine guanine phosphoribosyl transferase (HPRT)	Hprt	AGGGATTTGAATCATGTTTGTGTCA
Serine (or cysteine) peptidase inhibitor, claude E, member1 (PAI; plasminogen activator inhibitor-1)	Serpine1	CCTCATCCTGCCTAAGTTCTCTCTG
Coagulation Factor 3 (TF; Tissue Factor)	F3	AGAGTGTCCTGGGAGAAACACTCAT
Heme Oxygenase-1 (HO-1)	Hmox1	AAGGCTTTAAGCTGGTGATGGCCTC
Chemokine (C-X-C motif) ligand 2 (MIP-2; macrophage inflammatory protein-2; MIP-2)	Cxcl2	CCAAAAGATACTGAACAAAGGCAAG
Tumor Necrosis Factor alpha (TNF-α)	Tnf	TCACACTCAGATCATCTTCTCAAAA
Endothelin 1 (ET-1)	Edn1	GCTCCAGAAACAGCTGTCTTGGGAG
Endothelin Receptor A (ETA)	Ednra	GTGTTTAAGCTGTTGGCGGGGCGCT
Endothelin Receptor B (ETB)	Ednrb	GCCTACAAGCTGCTGGCAGGGGACT

*Gene symbols are as provided by the Applied Biosystems Incorporated.
**Target probe sequence represents the sequence which will hybridize with the PCR product and it is labeled such that once hybridized its fluorescence levels will be quantitatively detected by the ABI 7900 HT Sequence Detection System (Applied Biosystems). The forward and reverse primer sequences are proprietary and not provided by Applied Biosystems Inc.

ISSN: 1743-8977