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Figure 2 | Particle and Fibre Toxicology

Figure 2

From: Characterization of immortalized MARCO and SR-AI/II-deficient murine alveolar macrophage cell lines

Figure 2

Growth rate of the continuous murine alveolar macrophage single cell clones ZK1 (), ZK2 (■) and ZK6 (▲). Cells were seeded in six-well plate at 2 × 104 cells/ml and incubated at 37°C in a 5% CO2-humidified atmosphere in RPMI/10% FBS complete medium. Three wells per clone were harvested with cold PBS at 14 h and 18 h post seeding, and cells from each well were counted by a hemocytometer with trypan blue exclusion of dead cells. The obtained average cell count for each clone at each time point was plotted against the time. Doubling time (mean generation time = mgt) was calculated according the formula: N = N02T/mgt. N is the number of cells at any time T; N0, is the number of cells at an initial point. The doubling time of ZK cell lines is approximately 14 hours.

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