Figure 6

(A) ZK cell lines and primary AMs with MS^-/- significantly diminished phagocytosis of fluorescent latex beads. Wild type primary AMs were used as control. Values shown are the means ± SD from four separate experiments. **Significant difference from WT control, P < 0.001. (B) Polyinosinic acid (PolyI) had no effect but dextran sulfate (DS) increased binding by ZK1 cells. In contrast, PolyI and DS marked reduced wild-type AM binding of the latex beads. Data are expressed as the mean ± SD and compared to the control in each group (WT, MS^-/-, ZK1, respectively). *Significant difference compared with ZK1 (P < 0.05); **Significant difference compared with WT (P < 0.001). PolyI and DS (500 kDa), 10 μg/ml each. (C) The inhibition of ZK1 cells binding of the latex beads by dextran sulfate was size-dependent. Only smaller 5-8-kDa dextran sulfate was able to inhibit ZK1 cells binding of the latex beads. Data were shown as means ± SD. * P < 0.05 versus control (n = 4). DS, 10 μg/ml.