Diesel exhaust increases EGFR and phosphorylated C-terminal Tyr 1173 in the bronchial epithelium

Table 2 Antibodies used for immunohistochemical staining

Antibody	Clone or Source	Dilution	Specificity against	Source
EGFR	H11Mouse	1:140	EGFR	Dako Glostrup, Denmark
P-Tyr 845	Rabbit	1:45	Phosphorylated Tyr 845on EGFR	Cell Signaling Technology, MA, USA
P-Tyr 992	Rabbit	1:30	Phosphorylated Tyr 992on EGFR	Cell Signaling Technology, MA, USA
P-Tyr 1068	1H12Mouse	1:45	Phosphorylated Tyr 1068on EGFR	Cell Signaling Technology, MA, USA
P-Tyr 1110	Rabbit	1:30	Phosphorylated Tyr 1110on EGFR	Santa Cruz Biotechnology, Santa Cruz, CA, USA
P-Tyr 1173	Rabbit	1:60	Phosphorylated Tyr 1173on EGFR	Santa Cruz Biotechnology, Santa Cruz, CA, USA
P-Tyr 416	Rabbit	1:45	Phosphorylated Tyr 416on Src family	Cell Signaling Technology, MA, USA
P-MEK 1, 2	Rabbit	1:40	Phosphorylated Ser 217/221	Cell Signaling Technology, MA, USA
P-ERK 1, 2	Rabbit	1:40	Phosphorylated Thr 202 and Tyr 204 of human ERK	Cell Signaling Technology, MA, USA

ISSN: 1743-8977