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Table 2 Antibodies used for immunohistochemical staining

From: Diesel exhaust increases EGFR and phosphorylated C-terminal Tyr 1173 in the bronchial epithelium

Antibody Clone or Source Dilution Specificity against Source
EGFR H11Mouse 1:140 EGFR Dako Glostrup, Denmark
P-Tyr 845 Rabbit 1:45 Phosphorylated Tyr 845on EGFR Cell Signaling Technology, MA, USA
P-Tyr 992 Rabbit 1:30 Phosphorylated Tyr 992on EGFR Cell Signaling Technology, MA, USA
P-Tyr 1068 1H12Mouse 1:45 Phosphorylated Tyr 1068on EGFR Cell Signaling Technology, MA, USA
P-Tyr 1110 Rabbit 1:30 Phosphorylated Tyr 1110on EGFR Santa Cruz Biotechnology, Santa Cruz, CA, USA
P-Tyr 1173 Rabbit 1:60 Phosphorylated Tyr 1173on EGFR Santa Cruz Biotechnology, Santa Cruz, CA, USA
P-Tyr 416 Rabbit 1:45 Phosphorylated Tyr 416on Src family Cell Signaling Technology, MA, USA
P-MEK 1, 2 Rabbit 1:40 Phosphorylated Ser 217/221 Cell Signaling Technology, MA, USA
P-ERK 1, 2 Rabbit 1:40 Phosphorylated Thr 202 and Tyr 204 of human ERK Cell Signaling Technology, MA, USA