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Figure 5 | Particle and Fibre Toxicology

Figure 5

From: Surface area of particle administered versus mass in determining the pulmonary toxicity of ultrafine and fine carbon black: comparison to ultrafine titanium dioxide

Figure 5

A comparison of cytotoxicity of low, medium and high doses UFTiO 2 (Panel A) and UFCB (Panel B) at the various post-exposure time points. Comparison of cytotoxicity of low, medium and high doses UFTiO2 (Panel A) and UFCB (Panel B) at the various post-exposure time points. Rats were exposed to various mass doses of UFTiO2 (0.26, 0.52, and 1.04 mg/rat) and UFCB (0.047, 0.094, and 0.188 mg/rat) by intratracheal instillation. These mass doses resulted in identical surface area doses (0.0313, 0.0625, and 0.125 cm2/cm2) of UFTiO2 and UFCB. Animals were euthanized at 1 day, 7 days, and 42 days post-exposure and bronchoalveolar lavage was conducted. Cytotoxicity was assessed by measuring LDH activity in BALF. Control values of LDH activity for the UFTiO2 study were 46.375 ± 2.24, 39.5 ± 1.35 and 37.25 ± 2.63 and for the UFCB study were 49.375 ± 1.46, 43.75 ± 0.840 and 45.125 ± 1.69 at 1, 7 and 42 days, respectively. Values are the increases in LDH activity above the BALF control and are given as means ± SEM of 8 rats. * indicates that LDH activity for that group was significantly higher than control groups (p < 0.05) using ANOVA.

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