Lung inflammation and genotoxicity following pulmonary exposure to nanoparticles in ApoE-/- mice

Table 2 Expression (mRNA) of Mip-2, Mcp-1 and Il-6 in lung tissue and cell distribution and protein in BAL fluid 24 h after inhalation or instillation of carbon black in ApoE^-/- mice.

		Control	Low dose	High dose	significant dose-related differences ^b
Inhalation		HEPA air 1/2–1 1/2 h	CB 60 mg/m ³ , 1/2 h	CB 60 mg/m ³ , 1 1/2 h
lung tissue	*Mip-2*	9.9 ± 1.6	11.6 ± 2.9	17.6 ± 3.1	High dose ≈ Low dose
	*Mcp-1*	44.4 ± 10.5	97.2 ± 24.8*	79.9 ± 18.7	Low dose ≈ High dose
	*Il-6*	3.4 ± 0.6	4.3 ± 0.9	3.2 ± 0.5	Low dose ≈ High dose
BAL	Neutrophils% ^a	1.1 ± 0.4	0.7 ± 0.3	5.6 ± 3.2	High dose ≈ Low dose
	Macrophages% ^a	97.5 ± 0.3	97.9 ± 0.2	92.5 ± 4.1	High dose ≈ Low dose
	Total BAL cells	54750 ± 4891	66567 ± 6304	77867 ± 4896
	Protein	91.2 ± 3.7	108.1 ± 7.5*	118.5 ± 5.5**	High dose ≈ Low dose
Instillation		Vehicle control	CB 18 μg	CB 54 μg
lung tissue	*Mip-2*	5.1 ± 0.5	37.1 ± 13.4***	134.8 ± 33.2***	High dose>>>Low dose
	*Mcp-1*	28.3 ± 2.7	511.2 ± 246.7***	1087 ± 310.6***	High dose ≈ Low dose
	*Il-6*	1.1 ± 0.1	14.3 ± 7.2***	44.0 ± 13.0***	High dose>Low dose
BAL	Neutrophils% ^a	5.3 ± 1.6	41.3 ± 10.2*	75.8 ± 3.4***	High dose ≈ Low dose
	Macrophages% ^a	93.6 ± 1.5	57.7 ± 10.2***	22.1 ± 3.7***	High dose>>>Low dose
	Total BAL cells	65290 ± 5246	88173 ± 19861	78596 ± 21414
	Protein	110.6 ± 5.7	150.4 ± 7.7***	182.4 ± 7.1***	High dose>Low dose

Results are given as mean ± SEM. Expression of mRNA is normalized to 18S rRNA and is multiplied by 10⁷. The effect of exposure was tested by nested ANOVA tests with the dose of CB nested in the method of exposure. Normal distributions of the residuals of nested ANOVA tests were assessed by the Kolmogorov-Smirnov tests with 5% as significance level. ^a:Due to uneven variance, we determined 95; 99 and 99.9% confidence interval for means. ^b ≈ , >, >>, >>> indicate statistical significance p > 0.05, p < 0.05 p < 0.01 and p < 0.001, respectively. *, **, *** refer to statistical significance p < 0.05, p < 0.01 and p < 0.001, respectively. Statistical difference by method of exposure: Mip-2, p ≈ 0.08; Mcp-1, p < 0.001; Il-6, p < 0.05; Neutrophils%, p < 0.01; macrophages%, p < 0.001; Protein, p < 0.001;

ISSN: 1743-8977