Table 4 Expression (RNA) of Mip-2, Mcp-1 and Il-6 in lung tissue and cell distribution, DNA damage by the comet assay and protein in BAL fluid 3 and 24 h instillation of negatively (QD620) and positively (QD621) charged CdTe quantum dots in ApoE-/- mice.
From: Lung inflammation and genotoxicity following pulmonary exposure to nanoparticles in ApoE-/- mice
| Â | Â | Vehicle | QD620 | QD621 | significant dose-related differences b |
|---|---|---|---|---|---|
3 h | Â | Â | Â | Â | Â |
lung tissue | Mip-2 a | 36.0 ± 8.0 | 355.9 ± 38.3*** | 488.6 ± 188.4*** | QD621 ≈ QD620 |
|  | Mcp-1 | 20.4 ± 2.7 | 246.2 ± 61.7*** | 416.6 ± 127.6*** | QD621 ≈ QD620 |
|  | Il-6 | 13.3 ± 0.8 | 172.0 ± 30.4*** | 341.0 ± 97.3*** | QD621 ≈ QD620 |
BAL | Neutrophils % a | 6.8 ± 2.2 | 11.1 ± 6.0 | 10.8 ± 4.2 | QD620 ≈ QD621 |
|  | Macrophages % a | 91.8 ± 2.1 | 87.6 ± 6.2 | 86.4 ± 5.0 | QD620 ≈ QD621 |
|  | Total BAL cells | 54120 ± 6853 | 28965 ± 3017 | 34034 ± 3195 |  |
|  | Comet %T | 9.0 ± 1.1 | 29.3 ± 3.3*** | 29.7 ± 2.9*** | QD621 ≈ QD620 |
|  | Protein | 131.2 ± 8.6 | 129.4 ± 4.0 | 153.2 ± 10.5 | QD621 ≈ QD620 |
24 h | Â | Â | Â | Â | Â |
lung tissue | Mip-2 | 33.4 ± 14.4 | 848.4 ± 205.1* | 920.7 ± 148.8** | QD621 ≈ QD620 |
|  | Mcp-1 | 152.2 ± 43.5 | 4471.9 ± 1613*** | 5956.3 ± 817.5*** | QD621 ≈ QD620 |
|  | Il-6 | 6.5 ± 1.6 | 1114 ± 471.6*** | 1626.7 ± 531.6*** | QD621 ≈ QD620 |
BAL | Neutrophils % a | 20.6 ± 9.1 | 93.2 ± 2.1*** | 97.1 ± 0.7*** | QD621 ≈ QD620 |
|  | Macrophages % a | 73.3 ± 8.3 | 5.7 ± 1.4** | 2.6 ± 0.6** | QD621 ≈ QD620 |
|  | Total BAL cells | 63360 ± 7420 | 199600 ± 44198 | 308000 ± 101621 |  |
|  | Protein | 223.4 ± 29.6 | 560 ± 32.8*** | 675.8 ± 80.3*** | QD621 ≈ QD620 |