Figure 1

Identification of isolated AT-II cells with phosphine 3 R fluorescent dye. (A) A representative image from CD-exposed rat showing the staining of AT-II cell with bright green color. Note the presence of a hypertrophied AT-II cell (white arrow) that is larger in size than the other cells (blue arrows). Cells that are not AT-II cells (yellow arrow) do not get the stain. Reference bar is 20 μm. (B) An image of an AT-II cell captured under higher magnification demonstrating the lamellar bodies and the cells close to it do not have lamellar bodies and were classified as non AT-II cells. Reference bar is 20 μm. (C) A representative electron microscope image of an AT-II cell showing the lamellar bodies (black arrow heads). Reference bar is 2 μm.