Figure 3

Laser scanning micrographs of occludin in EC, MDM and MDDC. The occludin arrangement in 16HBE14o-, MDM and MDDC mono-cultures visualized with LSM. Control 16HBE14o- cells (A) and DEP exposed 16HBE14o- cells (B) were labelled for F-actin (turquoise) and occludin (red). For F-actin no qualitative difference was found between control and treated cells. However, the iso-surface presentation of occludin in control cells (A') showed a more structured TJ occludin belt compared with DEP treated cells (B") which shows a predominant localization of occludin in the cytoplasm. Occludin was visualized in mono-cultures of MDM (D, control; C, DEP treatment) and MDDC (E, control; F, DEP treatment). Cells were stained for F-actin (in MDM white, in MDDC black and occludin (shown in red). MDM and MDDC expressed the TJ protein occludin. After exposure to DEP for 24 h occludin signals were reduced (for MDM in D' and MDDC in F'). These are representative images and for all pictures the same settings were used.