Figure 4
From: Diesel exhaust particles modulate the tight junction protein occludin in lung cells in vitro
mRNA expression levels of occludin in EC, MDM and MDDC. Quantification of occludin mRNA expression in MDM, MDDC, and EC mono-cultures by qRT-PCR. Threshold of occludin was set by the threshold of the housekeeping gene β-actin in each sample (ΔCT). Expression levels of occludin are illustrated by the β-actin normalized concentrations (2-ΔCT × 15000). cDNA derived from MDM (grey columns), MDDC (black columns) and 16HBE14o- (white columns) control cells and cells treated with DEP (0.5 μg/ml, 5 μg/ml, 50 μg/ml, and 125 μg/ml). Values are presented as means ± SD; * p ≤ 0.05. All experiments were independently performed between 4-7 times.