Figure 2From: Ultrafine carbon particles down-regulate CYP1B1 expression in human monocytes Particle induced down-regulation of CYP1B1 is not due to contaminant LPS. CD14++ monocytes remained untreated (none) or were stimulated with LPS (10 ng/ml) and ultrafine P90 (32 μg/ml) each with and without 15 min PolymyxinB preincubation to suppress the LPS effect. After incubation for 3 h cells were lysed and mRNA levels were determined by RT-PCR (n = 6 incubations from different donors, mean ± S.D.; * p < 0.05 compared to untreated cells).Back to article page