Figure 3

A) Dose response analysis of ultrafine P90-induced down-regulation of CYP1B1. CD14++ cells remained untreated (0 μg/ml) or were stimulated with various doses of 0.32 μg/ml - 1 mg/ml of ultrafine P90 for 3 h. mRNA was recovered and levels of CYP1B1 mRNA were determined by RT-PCR (n = 5 incubations from different donors, mean ± S.D.; * p < 0.05 compared to 0 μg/ml). B) Time course of ultrafine P90-induced down-regulation of CYP1B1. CD14++ monocytes were stimulated for 0, 0.5, 1, 2, 3, 6 and 20 h with ultrafine P90 (32 μg/ml) (n = 3 incubations from different donors, mean ± S.D.; * p < 0.05 compared to 0 h).