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Figure 8 | Particle and Fibre Toxicology

Figure 8

From: A dose-controlled system for air-liquid interface cell exposure and application to zinc oxide nanoparticles

Figure 8

Effect of cell handling on cell viability during ALICE experiments. Cell viability (WST-1 test; 3 h incubation after exposure) was measured in immortalized human alveolar epithelial-like cells (A549). "No expo" cells were treated identical to ALICE cells except that they were not put into the ALICE and instead they remained in the incubator. Cells maintained under submerged conditions served as a control (100%). Cell viability was slightly impaired for all cases (87 ± 2%), but there was no statistically significant difference between 10 mM NaCl- and citrate-exposed cells and not exposed cells. Hence, the slightly reduced cell viability (relative to submerged conditions) is likely due to culturing the cells for about 18 h at the air-liquid interface prior to exposure, but not due to cell handing in the ALICE.

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