Figure 1

DEP and PAMP modulate mRNA expression of Scavenger Receptors in MDDC. MDDC were maintained in medium alone or activated with TLR2, -3 and -4 ligands (Pam₃CSK₄ (10 μg/ml), poly(I:C) (10 μg/ml) and LPS (1 μg/ml) respectively), associated or not with DEP (10 μg/ml) during 1 h (part A) and 3 h (part B). MDDC were harvested for mRNA isolation followed by measurement of CD36, CXCL16, LOX-1 and SR-B1 levels by quantitative RT-PCR. Results were expressed as the relative gene expression calculated for each experiment in folds (2^-(ΔΔCt)) compared to unstimulated cells used as calibrator. Data reported the mean ± SEM from 4 independent experiments. +: p < 0.05; ++: p < 0.01; +++: p < 0.001 compared with cells in medium alone. ✻: p < 0,05 compared with TLR-treated cells.